Product Info Summary
SKU: | A02682-2 |
---|---|
Size: | 100 µg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-RAB1A Antibody Picoband™
SKU/Catalog Number
A02682-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-RAB1A Antibody Picoband™ catalog # A06317-1. Tested in WB, FCM, ELISA applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-RAB1A Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02682-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Clone Number
1B9
Isotype
IgG
Immunogen
E.coli-derived human RAB1A recombinant protein (Position: R74-Q199).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A02682-2 is reactive to RAB1A in Human
Applications
A02682-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Observed Molecular Weight
23,22 kDa
Calculated molecular weight
22.678kDa
Background of RAB1A
Ras-related protein Rab-1A is a protein that in humans is encoded by the RAB1A gene. This gene encodes a member of the Ras superfamily of GTPases. Members of the gene family cycle between inactive GDP-bound and active GTP-bound forms. This small GTPase controls vesicle traffic from the endoplasmic reticulum to the Golgi apparatus. Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x106 cells, Human
ELISA, 0.1-0.5 µg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RAB1A using anti-RAB1A antibody (A02682-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB1A antigen affinity purified polyclonal antibody (Catalog # A02682-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB1A at approximately 23,22 kDa. The expected band size for RAB1A is at 23 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of A549 cells using anti-RAB1A antibody (A02682-2).
Overlay histogram showing A549 cells stained with A02682-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB1A Antibody (A02682-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For RAB1A (Source: Uniprot.org, NCBI)
Gene Name
RAB1A
Full Name
Ras-related protein Rab-1A
Weight
22.678kDa
Superfamily
small GTPase superfamily
Alternative Names
DKFZp564B163; member RAS oncogene family; RAB1A, member RAS oncogene family; ras-related protein Rab-1A RAB1A RAB1, YPT1 RAB1A, member RAS oncogene family ras-related protein Rab-1A|GTP binding protein Rab1a|RAB1, member RAS oncogene family|Rab GTPase YPT1 homolog|YPT1-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB1A, check out the RAB1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB1A Antibody Picoband™ (A02682-2)
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